Isolation and identification of indole-3-ethanol (tryptophol) from cucumber seedlings.
نویسندگان
چکیده
Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments. Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography. Identification of the growth promoter as indole-3-ethanol was achieved by mass spectrometry, thin layer and gas chromatography, and ultraviolet and visible spectroscopy, as well as by physiological characteristics.
منابع مشابه
Isolation of Indole-3-ethanol Oxidase from Cucumber Seedlings.
Previous work in this laboratory has shown that cucumber (Cucumis sativus L.) seedlings contain large amounts, relative to other indolic compounds, of extractable indole-3-ethanol (IEt); tracer studies have established that IEt is metabolized to IAA. We have now succeeded in isolating an enzyme from these seedlings which catalyzes the oxidation of IEt to indole-3-acetaldehyde (IAAld). The ident...
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Indoleethanol-(14)C was applied to intact cucumber seedlings and to hypocotyl segments. The presence of indoleacetic acid-(14)C in tissue extracts was demonstrated by thin layer radiochromatography. There was no evidence of conversion of indoleacetic acid to indoleethanol. It is suggested that the growth-promoting activity of indoleethanol is due to its conversion to indoleacetic acid.
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In a continuing study of the biosynthetic pathway and regulatory mechanisms governing indole-3-acetic acid (auxin) formation, we report the isolation and initial characterization of three distinct indole-3-acetaldehyde reductases from cucumber seedlings. These enzymes catalyze the reduction of indole-3-acetaldehyde to indole-3-ethanol with the concomitant oxidation of NAD(P)H to NAD(P)+. Two of...
متن کاملTryptophol, a plant auxin isolated from the marine sponge Ircinia spinulosa.
Tryptophol [2-(3-indolyl)-ethanol], a well-known constituent of terrestrial plants and microorganisms, has so far been isolated from tryptophan fermentations, plant seedlings, Aspergillus niger, Agrobacterium tumefaciens, Ceratocystis spp., and Rhizobium spp. (Buckingham, 1994). We have now isolated tryptophol from the marine sponge Ircinia spinulosa collected from the Aegean Sea. In addition t...
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Analysis of neutral and acidic ethyl acetate extracts from culture medium of Azospirillum brasilense 703Ebc by high-performance liquid chromatography (HPLC) and combined gas chromatography-mass spectrometry demonstrated the presence of indole-3-acetic acid (IAA), indole-3-ethanol, indole-3-methanol, and indole-3-lactic acid. IAA in media of 20 strains of A. brasilense and Azospirillum lipoferum...
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ورودعنوان ژورنال:
- Plant physiology
دوره 42 4 شماره
صفحات -
تاریخ انتشار 1967